Ultra Yield Flask™ Performance Data

• Flasks that function as disposable fermentors
• Replacement for Glass Flasks
• Fit all Standard Flask Clamps
• Sterile, disposable, single-use flasks from 125 mL to 2.5L
• Fully scalable results

Ultra Yield Flasks are easily adapted into microbial growth protocols, all standard flask clamps and available in 50mL, 100mL, 200mL and 1L working volume sizes.

Ultra Yield Flasks can grow cells for longer time and have better oxygenation than glass flasks. The Ultra Yield Flasks can be supplied with simple to use AitOtop seals that facilitate optimal gas exchange. All of our Ultra Yield Flasks are scalable up to 2.5L and are completely disposable and recyclable.

Study 1

Genetech has kindly provided data on how Ultra Yield Flasks™ and AirOtop™ Seals have revolutionized their processes. The data presented is a comparison of Origami™ Cells and BL21™ in standard glass flasks vs. Ultra Yield Flasks. Ultra Yield Flasks have led to easier purifications because the cells are still in a transitional growth period, not a stationary phase more quickly, which causes secondary proteins to be produced. This may lead to protein degradation, insoluble proteins and, worst of all, loss of proteins. The Ultra Yield Flasks do not exhibit these proteins because you are still over expressing your protein up until the time you remove your cells. Ultra Yield Flasks and AirOtop Seals are a product that save liters of media, are simple to use, are scaleable between 50mL-1L, cost-effective with higher yields and fit into all of your existing shakers.

• 1 A1 N1
• 2 A1 I
• 3 A1 NI
• 4 ADI
• 5 B2 NI
• 6 B2 I
• 7 B4 NI
• 8 B4 NI
• 1-8 Origami Cells
• *9 Fcg NI
• *10 Fcg I

Study 2

During a study by NAU using Ultra Yield Flaks and a new media formulation, E.coli cultures obtained growth densities of 20 OD(600) - or more than a 10X fold increase. Ultra Yield Flasks allowed for better aeration thereby resulting in maintenance of a lower pH over time, enabling better cell growth. Better cell growth, higher protein and DNA expression were achieved. Using this system, the expression of vaccinia thymidine kinase, a protein that had a limited yield of 0.25 mg/L under standard media and glass flasks, was elevated to 10 mg/L of culture with Ultra Yield Flasks and AirOtop Seals.

When the volume of culture was decreased to 250mL, higher cell densities were possible. After 30 h (at pH7.6) three separate cultures had an OD(600) of 79 was achievable.

Lane 1: Sigma VII Marker
Lane 2: 25ug total soluble protein from E.coli supematant
Lane 3: 25ug Ni-NTA purified protein from glass flasks at 0.8 mg/mL from culture at 15OD

Lane 1: Sigma VII Marker
Lane 2: 30ug Ni-NTA protein from Thomson flasks at 15 mg/mL from culture at 27 OD
Lane 3: 38ug Ni-NTA purified protein from Thomson Ultra Yield Flasks at 18 mg/mL from culture at 23 OD.