Increased DNA and Protein       Production

Flasks can be used as       Disposable Fermentors

Elevated Cell Production

Direct Replacement for Glass       Flasks

Sterile and Disposable

Scaleable Results Between Flasks

Ultra Yield Flasks™ can be used with 50 mL-1Liter of Media


AirOtop Seals		125mL - 500mL Small Flasks		New Brunswick™ Floor Models Hold 12 Large Flasks		New Brunswick™ Floor Models Hold 12 Large Flasks

Ultra Yield Flasks™ are an easily adaptable product to any lab doing E.Coli, Yeast, & Microbial growth in flasks. Ultra Yield Flasks fit in all standard flask clamps.This means that no change of equipment is required. Ultra Yield Flasks have been used instead of fermentors because of their reasonable price and High Protein or DNA production. Ultra yield Flasks have also replaced Glass Flasks with up to 4X the growth capacity. One does not have to worry about shattering a flask or injuring one's self on broken glassware. Ultra Yield flasks have a growth life cycle of 50 hours at 37*C or an OD of 80, whereas standard flasks go into stationary phase at around 12-14hrs. Ultra Yield flasks allow you to take out your culture the next day at your convience, instead of having to pass the paper boy in the night to stop cellular death. Ultra Yield Flasks use the AirOtop™ seals to allow for optimized exchange of Oxygen and Carbon Dioxide. The AirOtop seals are easy to use. Just leave the tab (with liner) for quick checks of growth, inductions, etc. It is recommended you use the smaller Ultra Yield flasks for expression testing, and for starter cultures for improved growth. All of our Ultra Yield Flasks allow for scaleable data from our 125ml-2.5L range.

Ultra Yield Flasks like any lab advancement need to be used in the proper way. Shakers must have the the ability work at higher speeds. The optimum Orbit for all Ultra yield Flasks is best at 1.00 inch and circular orbit. We have found the New Brunswick 44™ Stackable does not work well if it is not balanced correctly. Check with your New Brunswick service person.

Instructions.
Ultra Yield Flasks can be used in many applications. Below we will list all recommended speeds, and other
factors key to your success with the Ultra Yield Flasks.

Recommended Speeds and Media amount for shaker with 1inch orbit.

Part# Media (mL)/ Flask Shaker Speed
931147 125mL Ultra Yield Flasks 50/case--Sterile Please cover the flask with AirOtop seals 899415	40-50mL/flask	300-350 RPM
931144 250mL Ultra Yield Flasks 50/case --Sterile Please cover the flask with AirOtop seals 899415	75-110mL /flask	300-350 RPM
931141 500mL Ultra Yield Flasks 25/case --Sterile Please cover the flask with AirOtop seals 899415	125-200mL/flask	300-350 RPM
931136-B 2.5L Ultra Yield Flasks--Sterile Please cover the flask with AirOtop seals 899414	500mL (optimum)	350-400 RPM
931136-B (Fermentor Mode)	1L	250-300 RPM

AirOTop Seals
899414	This Seal fits the 2.5L Ultra yield Flask 100/pack
899415	This Seal fits all Ultra Yield Flasks 125mL-500mL. It is a Universal fit on all standard Flasks 100/pack

Ultra Yield Flask vs Standard Flasks Estimated cost

Category Ultra Yield Flask		Standard Flasks
Typical Media needed	1L	Between 2L-4L to be equal
Typical cost of Media	$12.00/L-$20.00/L	$24.00/L-$48.00/L
Cost of lab tech making media based on $40.00 with Social Security, healthcare, FICA, unemployment taxes, pension,etc. Note at large Pharma this is around $84.00 with all overhead.	$40.00-$84.00	$40.00-$84.00
Chance of losing culture, and having to restart experiment and incurring line 1 and 2. Soap in flask, breakage, or other problems.	0%	15%
Cost of purification in lab tech time and materials.	$80.00-168.00	$320.00-$672.00
Cost of Flask Cleanup; energy, overhead, labor. Which breaks down into Social security, health care, FICA, unemployment comp taxes, pension, etc.	Recyclable Plastic #5	$25.00-$54.00

The cost of the plastic flask becomes INCIDENTAL due to of the overhead saved in solvent and time
because of the efficiency of the flask's higher product yield.

Genentech has kindly provided data on how Ultra Yield Flasks™ and AirOtop™ Seals have revolutionized their processes. The data presented is a comparison of Origami™ Cells, and BL21™ in standard glass flasks vs Ultra Yield Flasks. Ultra Yield Flasks have led to easier purifications because the cells are still in a transitional growth period, not a stationary phase. Please notice the lack of background proteins in the gel of the Ultra Yield Flasks. Standard glass flasks lead to stationary phase more quickly, which, causes secondary proteins to be produced. This may lead to protein degradation, insoluble proteins, and worst of all loss of proteins. The Ultra Yield Flasks do not exhibit these proteins because you are still over expressing your protein up until the time you remove your cells. Ultra Yield Flasks and AirOtop Seals are a product that save liters of media, are simple to use, are scaleable between 50mLs-1L, cost effective with higher yields, and fit into all of your existing shakers.


Standard 2L Shake Flask w/ 1Liter Ultra Yield 2.5L Flask w/1Liter

Old Standard Shake flask ULTRA YIELD Flask

HOURS

GSK has kindly provided data on how Ultra Yield Flasks and AirOtop Seals have revolutionized their processes. The comparison of the different flasks show the Ultra Yield Flasks give a higher cell density, lower acetate content for further fermentation steps, balanced pH, and cells that are not in stationary phase of growth. Ultra Yield Flasks donít extend log phase growth but add a new transition state allowing for growth up to 50 hours. Standard glass flasks only allow for a 10-12 hour window of growth phase and then go into stationary phase. This leads to cells being taken out quickly after overnight cultures because cells start to chew up the proteins for food and cause insoluble protein complexes. Ultra Yield Flasks maintain pH, which is extremely important due to the fact that below pkA of 6.0-6.1 many amino acids have lowered activity. Thus hindering the systems for protein replication. Ultra Yield Flasks and AirOtop Seals are a product that save liters of media, are simple to use, are scaleable between 50mLs-1L, cost effective with higher yields, and fit in all of your existing shakers.

Ultra Yield Flasks 10X Increase in Protein Expression

NAU has kindly provided data on how Ultra Yield Flasks™ and AirOtop™ Seals have allowed them to do crystallography and calorimetry. Ultra Yield Flasks have provided an opportunity to increase the growth capacity of shake flasks to a capacity rivaling fermentors. Ultra Yield Flasks and a new media formulation E.coli cultures obtained growth densities of 20 OD(600) or more than a 10X fold increase. A comparison of generic glass flasks and Thomson Ultra Yield Flasks demonstrated enhanced bacterial growth and increased protein yields in the protein expression of soluble vaccinia thymidine kinase.


When the volume of culture was decreased to 250 mL, higher cell densities were possible. After 30 h (at pH 7.6) three separate cultures had an OD(600) of at least 40, and after 49 h, an OD(600) of 79 was achievable.	Gel 1: Lane1: Sigma VII Marker Lane 2: 25 µg total soluble protein from E.coli supernatant Lane 3: 25 µg Ni-NTA purified protein from glass flasks at 0.8 mg/mL from culture at 15 OD	Gel 2: Lane1: Sigma VII Marker Lane 2: 30 µg Ni-NTA purified protein from Thomson flasks at 15 mg/mL from culture at 27 OD Lane 3: 38 µg Ni-NTA purified protein from Thomson Ultra Yield flasks at 18 mg/mL from culture at 23 OD

These results developed from Ultra Yield Flasks, AirOtop Seals, and SRAM media have produced higher densities of cells than previously possible in standard shake flasks. Ultra Yield Flasks allowed for better aeration, thereby resulting in maintenance of a lower pH over time, and enabling better cell growth. Better cell growth, higher protein and DNA expression were achieved. Using this system the expression of vaccinia thymidine kinase, a protein that had a limited yield of 0.25 mg/L under standard media and glass flasks was elevated to 10 mg/L of culture with Ultra Yield Flasks and AirOtop Seals.

Thomson Instrument Company • Phone: 800.541.4792 or 760.757.8080 e-mail: folks@htslabs.com Thomson Instrument Company is not affiliated with ORgami Cells E.Merck® is registered to EMD Chemicals, Inc. , BL21 from Invitrogen , New brunswick Scientific, Genentech, GSK, and NAU